Cambridge University has existing plans to conduct single-cell CITE-Seq profiling of bone marrow CD34+ cells taken from samples from young and aged adults for the Human Cell Atlas project. Our pilot project aims to produce complementary single-cell ATAC-Seq data from cells taken from the same samples.
The bone marrow CD34+ cells will be taken from 4 healthy young male adults and 4 aged male adults in overall good health. The chromatin organization will be analyzed by using the single-cell ATAC-Seq technique to assess the epigenetic changes through the aging process.
The project is currently under negotiations.